5. No one in your family bothers to ask you what you’re studying anymore.
The longer you spend in a Ph.D. program the more opportunities your family has to ask you what you’re doing in school. No doubt, you’ve spent the first couple years of graduate school going to family functions and trying to explain to your grandma what a molecule is. She will eventually come up with an explanation of what you are doing that she can share with her friends. Her description of your work may or may not be correct, but she’s not going to bother trying to understand it anymore. “Good for your honey, you’re so smart!”
4. Your former life as a bartender or grocery store clerk starts to sound really appealing. Continue Reading »
Posted in Careers, Fun things | Tagged grad school, Ph.D., ready to graduate, thesis committee, thesis defense | Leave a Comment »
In vitro fertilization (IVF) is a technology involving fertilization of an egg by a sperm cell outside the body in a laboratory dish. The origin of the term in vitro, which literally means in glass, dates back to the era when glass containers such as test tubes or petri dishes were popular for cultivating tissues outside the living organism it originated from. In recent times, when plastic has replaced glass, the term in vitro refers to any biological procedure that is performed outside the living organism to distinguish it from an in vivo procedure, where the tissue remains inside the living organism in its native environment (1). Continue Reading »
Posted in News | Tagged IVF, mitochondrial DNA, mitochondrial donor, three-parent dna testing | Leave a Comment »
Printing has been an integral part of society since the first movable type printing press was invented by Johannes Gutenberg in the 15th century. And yet, many recent headlines have heralded its impending death. As everything from books to newspapers transitioned to digital media, the traditional printing techniques seemed destined for the history books and museums. However, even before the popularity of printing started to fade, some of the concepts were being reimagined in amazing, new—and three dimensional— ways. Continue Reading »
Posted in News | Tagged 3D printing, bio inks, bioprinting, Printing tissue | Leave a Comment »
In this BioCompare video, Promega scientist, Dr. Keith Wood, explains how the smaller, brighter and sensitive NanoLuc® Luciferase allows scientists to observe protein behaviors inside cells, including rare events. See how a luciferase can now be used to investigate protein activities as well as for traditional luciferase genetic reporter assays.
Posted in Bioluminescence | Tagged BRET, living cells, NanoLuc Luciferase, protein interactions | 1 Comment »
Although previous references have provided data regarding the potential oncogenic role of the gene ETV7, there has been minimal investigation as to its physiological role.
In the following reference, Quintana, A. et al. (2014) Disease Models & Mechanisms 7, 265–70, zebrafish were used as in vivo model system to characterize ETV7.
One key experiment required the morpholino-oligonucleotide -mediated knockdown of in vivo ETV7. Two independent morpholinos were designed: one that inhibited translation and the other that inhibited proper splicing of exon 3. The efficacy of the translation –blocking morpholino was assessed with cell free expression of ETV7-tagged with hemagglutinin (HA).
Western blot performed with anti-HA antibodies determined the extent of the knockdown compared to a control containing no morpholino added. Once an efficient design was determined via cell-free expression screening, it was used for in vivo experiments. In conjunction additional other techniques, concluded that ETV7 is essential for normal red blood cell development.
Posted in Cell-free expression, General | Tagged cell-free expression, oligo-mediated knockdown, zebrafish | Leave a Comment »
Paul Steyn has posted an amazing series of photographs taken by Adam Riley in Hemis National Park in the Himalayas on the National Geographic News web site. These photographs are the first photo documentation of a successful snow leopard hunt, and underscore the amazing biology of this area. If you are interested in learning more, we have another blog post about early work to isolate induced pluripotent stem cells from adult animals–an attempt to compliment the habitat preservation and other efforts to save this incredible hunter before it truly becomes a ghost.
Posted in News | Tagged Adam Riley, Himalayas, snow leopard kill, snow leopards | 1 Comment »